The diluted cultures and real drinking water samples were tested by the microarray with 100% accuracy. The results were specific and reproducible, with a detection sensitivity of 0.1 ng DNA or 10 4 CFU/ml achieved for pure cultures of each target organism. Testing was carried out against a total of 218 bacterial strains, including 53 representative strains, 103 clinical isolates and 62 strains of other bacterial species belonging to 10 genera and 48 species.
This new diagnostic contains 26 specific probes and can simultaneously detect Aeromonas hydrophila, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Staphylococcus aureus, Vibrio choleraeo, Vibrio parahaemolyticus, Yersinia enterocolitica and Leptospira interrogans. Published online ahead of print on as DOI 10.1099/ ijs.0.63722-0. Evolutionary distances were calculated using the Jukes & Cantor evolutionary model and a phylogenetic tree was Abbreviation: rep-PCR, repetitive-element polymerase chain reaction. Therefore, we have developed an oligonucleotide-based microarray using the sequences of 16S–23S rDNA internal transcribed spacer regions (ITS) and the gyrase subunit B gene ( gyrB) found in the most prevalent and devastating waterborne pathogenic agents. from GenBank and edited by using the BioEdit program (Hall, 1999) and ForCon (Raes & Van De Peer, 1999). Consumption of water contaminated with infectious agents, toxic chemicals or radiological hazards represents a significant health risk and is strongly associated with mortality. The safety and accessibility of drinking water are major concerns throughout the world.